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Phenol Red Carbohydrate Broth (BAM M121)

[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]

Phenol Red Carbohydrate Broth

(BAM M121)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................1.0g

Carbohydrate solution................................. 200.0mL

Phenol Red solution........................................ 7.2mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostics.

Phenol Red Solution:

Composition per 10.0mL:

Phenol Red......................................................0.025g

Preparation of Phenol Red Solution: Add Phe

nol Red to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly.

Carbohydrate Solution:

Composition per 200.0mL:

Carbohydrate..............................................5.0–10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate (5.0g dulcitol, 10.0g lactose, or 10.0g

sucrose) to distilled/deionized water and bring vol

ume to 200.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 800.0mL. Mix thoroughly.

Adjust pH to 7.4 if necessary. Distribute into tubes

containing an inverted Durham tube. Fill each tube

with 8.0mL of medium. Autoclave for 15 min at 13 psi

pressure–118°C. Cool to 45°–50°C. Aseptically add

2.0mL of sterile carbohydrate solution to each tube.

Use: For the determination of fermentation reactions

in the differentiation of microorganisms, such as Sal

monella spp. Fermentation is determined by the pro

duction of acid—broth turns yellow—and formation

of gas—bubble trapped in Durham tube.