培養基配方

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Phenol Red Broth

[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]

Phenol Red Broth

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................10.0g

NaCl....................................................................5.0g

Phenol Red......................................................0.018g

Carbohydrate solution................................... 20.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 20.0mL:

Carbohydrate..................................... 5.0–10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 980.0mL. Mix thoroughly.

Adjust pH to 7.4 if necessary. Distribute into tubes

containing an inverted Durham tube. Fill each tube

with 9.8mL of medium. Autoclave for 15 min at 13 psi

pressure–118°C. Cool to 45°–50°C. Aseptically add

0.2mL of sterile carbohydrate solution to each tube.

Use: For the determination of fermentation reactions

in the differentiation of microorganisms. Fermenta

tion is determined by the production of acid—broth

turns yellow—and formation of gas—bubble trapped

in Durham tube.