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Pectin Medium

[所屬分類:培養基配方] [發布時間:2021-12-8] [發布人:網站管理員2] [閱讀次數:] [返回]
Pectin Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pectin ................................................................30.0g
Yeast extract........................................................5.0g
Bromthymol Blue (0.1% solution).................. 1.0mL
CaCl2·2H2O (10.0% solution)......................... 0.6mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add 100.0mL of dis
tilled/deionized water to a 2.0L flask and place on a
magnetic stirrer with no heat. While stirring, slowly
add CaCl2·2H2O, Bromthymol Blue solution, yeast
extract, and pectin. Add slowly to ensure each parti
cle is wetted. Gently heat and bring to almost boiling.
Adjust pH to 7.3 with 1N NaOH. Do not overshoot
pH 7.3.
Use: For the isolation and presumptive identification
of Yersinia enterocolitica and Yersinia pseudotuber
culosis from other fermenting Gram-negative bacilli
such as Enterocolitica agglomerans which is often
confused with Yersinia. Enterocolitica agglomerans
does not produce pectinase. Yersinia enterocolitica is
strongly positive for pectinase activity. Yersinia
pseudotuberculosis is weakly positive for pectinase
activity. Yersinia pestis is negative for pectinase ac
tivity. Also used for the differentiation of Klebsiella
oxytoca, which is pectinase positive.
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