99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

<bdo id='3xZRH'><sup id='Poeja'><div id='8OuhD'><bdo id='Nw8XJ'></bdo></div></sup></bdo>

培養基配方

您現在的位置：網站首頁 >> 培養基配方

PE2 Medium

[所屬分類:培養基配方] [發布時間:2021-12-8] [發布人:網站管理員2] [閱讀次數:] [返回]

PE2 Medium

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Peptone .............................................................20.0g

Yeast extract........................................................3.0g

Alaska seed peas ..........................................416–520

Bromcresol Purple solution ............................ 2.0mL

Bromcresol Purple Solution:

Composition per 100.0mL:

Bromcresol Purple ..............................................2.0g

Ethanol.......................................................... 10.0mL

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to 10.0mL of ethanol. Mix

thoroughly. Bring volume to 100.0mL with distilled/

deionized water. Filter sterilize.

Preparation of Medium: Add components, ex

cept Alaska seed peas, to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat until dissolved. Add 8–10 Alaska seed peas to

each of 18 × 150 mm screw-capped tubes. Distribute

the broth into each tube in 19.0mL volumes. Allow

tubes to stand for 1 hr. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation of Clostridium botulinum

from foods.