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PALCAM Agar (Polymyxin Acriflavine LiCl Ceftazidime Esculin Mannitol Agar) (PALCAM Listeria Selective Agar) (BAM M118a)

[所屬分類:培養基配方] [發布時間:2021-12-8] [發布人:網站管理員2] [閱讀次數:] [返回]
PALCAM Agar
(Polymyxin Acriflavine LiCl
Ceftazidime Esculin Mannitol Agar)
(PALCAM Listeria Selective Agar)
(BAM M118a)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Peptone .............................................................23.0g
LiCl...................................................................15.0g
Agar ..................................................................10.0g
Mannitol............................................................10.0g
NaCl....................................................................5.0g
Yeast extract........................................................3.0g
Starch ..................................................................1.0g
Esculin ................................................................0.8g
Ferric ammonium citrate.....................................0.5g
Glucose ...............................................................0.5g
Phenol Red........................................................0.08g
PALCAM selective supplement.................... 10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
PALCAM Selective Supplement:
Composition per 10.0mL:
Ceftazidime....................................................20.0mg
Polymyxin B ..................................................10.0mg
Acriflavine·HCl................................................5.0mg
Preparation of PALCAM Selective Supple
ment: Add components to distilled/deionized water
and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Egg Yolk Emulsion:
Composition:
Chicken egg yolks..................................................11
Whole chicken egg...................................................1
Preparation of Egg Yolk Emulsion: Soak eggs
with 1:100 dilution of saturated mercuric chloride so
lution for 1 min. Crack eggs and separate yolks from
whites. Mix egg yolks with 1 chicken egg.
Preparation of Medium: Add components, ex
cept PALCAM selective supplement, to distilled/
deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile PALCAM selec
tive supplement. Mix thoroughly. The addition of
25.0mL of egg yolk emulsion may aid in the recovery
of damaged Listeria. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the selective isolation, cultivation, and dif
ferentiation of Listeria monocytogenes and other
Listeria species from foods.
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