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Pai Medium

[所屬分類:培養基配方] [發布時間:2021-12-8] [發布人:網站管理員2] [閱讀次數:] [返回]
Pai Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Homogenized whole egg ............................ 666.0mL
NaCl (0.85% solution)................................ 334.0mL
pH 6.75 ± 0.2 at 25°C
Homogenized Whole Egg:
Composition per liter:
Whole eggs ...................................................... 18-24
Preparation of Homogenized Whole Egg: Use
fresh eggs, less than 1 week old. Scrub the shells with
soap. Let stand in a soap solution for 30 min. Rinse in
running water. Soak eggs in 70% ethanol for 15 min.
Break the eggs into a sterile container. Homogenize by
shaking. Filter through four layers of sterile cheesecloth
into a sterile graduated cylinder. Measure out 1.0L.
Preparation of Medium: Combine components.
Mix thoroughly. Aseptically distribute into sterile
tubes. Inspissate tubes in a slanted position at 80°–
90°C (moist heat) for 30 min.
Use: For the maintenance of stock cultures of Salmo
nella typhi and other Salmonella species.
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