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Møller Decarboxylase Broth

[所屬分類:培養基配方] [發布時間:2021-12-2] [發布人:網站管理員2] [閱讀次數:] [返回]
Møller Decarboxylase Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Amino acid........................................................10.0g
Peptic digest of animal tissue..............................5.0g
Beef extract.........................................................5.0g
Glucose ...............................................................0.5g
Bromcresol Purple ............................................0.01g
Cresol Red .......................................................5.0mg
Pyridoxal..........................................................5.0mg
pH 6.0 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Use L-lysine, L-arginine, or L-ornithine. Mix thor
oughly. Gently heat until dissolved. Distribute into
screw-capped tubes in 5.0mL volumes. Autoclave
for 15 min at 15 psi pressure–121°C. A slight precip
itate may form in the ornithine broth.
Use: For the differentiation of Gram-negative enteric
bacteria based on the production of arginine dihydro
lase, lysine decarboxylase, or ornithine decarboxylase.
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