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培養基配方

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Modified Oxford Listeria Selective Agar (Oxford Agar, Modified) (Listeria Selective Agar, Modified Oxford) (MOX Agar) (BAM M103a)

[所屬分類:培養基配方] [發布時間:2021-12-2] [發布人:網站管理員2] [閱讀次數:] [返回]

Modified Oxford Listeria Selective Agar

(Oxford Agar, Modified)

(Listeria Selective Agar, Modified

Oxford)

(MOX Agar)

(BAM M103a)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1002mL:

Special peptone.................................................23.0g

LiCl...................................................................15.0g

Agar ..................................................................12.0g

NaCl....................................................................5.0g

Cornstarch...........................................................1.0g

Esculin ................................................................1.0g

Ferric ammonium citrate.....................................0.5g

Buffered colistin methane sulfonate

solution ..................................................... 1.0mL

Buffered moxalactam solution........................ 2.0mL

pH 7.2 ± 0.2 at 25°C

Buffered Colistin Methane Sulfonate

Solution:

Composition per 100.0mL:

Colistin methane sulfonate .................................1.0g

Potassium phosphate buffer, 0.1M. pH 6.0. 100.0mL

Preparation of Buffered Colistin Methane

Sulfonate Solution: Add colistin methane sul

fonate to 100.0mL 0.1M potassium phosphate buffer.

Mix thoroughly. Adjust pH to 6.0. Filter sterilize.

Store at –20°C.

Buffered Moxalactam Solution:

Composition per 100.0mL:

Sodium or ammonium moxalactam....................1.0g

Preparation of Buffered Moxalactam Solu

tion: Add sodium or ammonium moxalactam to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Filter sterilize. Store at –20°C.

Preparation of Medium: Graduatlly add compo

nents, except buffered moxalactam solution, to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Adjust pH to 7.2. Gently heat and bring

to boiling. Autoclave for 10 min at 15 psi pressure–

121°C. Cool quickly to 46°C. Aseptically add 2.0mL

of sterile moxalactam solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Listeria

monocytogenes from specimens containing a mixed

bacterial flora.

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Modified Oxford Listeria Selective Agar (Oxford Agar, Modified) (Listeria Selective Agar, Modified Oxford) (MOX Agar) (BAM M103a)