99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開

編號：
批號：

培養基配方

您現在的位置：網站首頁 >> 培養基配方

Modified Campylobacter Blood-Free Selective Agar Base (Modified Campylobacter Charcoal Differential Agar) (Modified CCDA) (BAM M30a)

[所屬分類:培養基配方] [發布時間:2021-12-2] [發布人:網站管理員2] [閱讀次數:] [返回]

Modified Campylobacter Blood-Free

Selective Agar Base

(Modified Campylobacter Charcoal

Differential Agar)

(Modified CCDA)

(BAM M30a)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1012mL:

Agar ..................................................................12.0g

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

NaCl....................................................................5.0g

Charcoal..............................................................4.0g

Casein hydrolysate..............................................3.0g

Yeast extract........................................................2.0g

Sodium deoxycholate..........................................1.0g

FeSO4 ................................................................0.25g

Sodium pyruvate...............................................0.25g

Cefoperazone solution .................................... 4.0mL

Amphotericin B solution................................. 4.0mL

Rifampicin solution......................................... 4.0mL

pH 7.4 ± 0.2 at 25°C

Cefoperazone Solution:

Composition per 10.0mL:

Cefoperazone ..................................................0.037g

Preparation of Cefoperazone Solution: Add

cefoperazone to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Rifampicin Solution:

Composition per 100.0mL:

Rifampicin ........................................................0.25g

Ethanol, absolute........................................... 50.0mL

Preparation of Rifampicin Solution: Add

rifampicin to 50.0mL of ethanol. Mix thoroughly.

Bring volume to 100.0mL with distilled/deionized

water. Filter sterilize.

Amphotericin B Solution:

Composition per 10.0mL:

Amphotericin B...............................................0.005g

Preparation of Amphotericin B Solution:

Add Amphotericin B to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize. Can be stored for 1 year at –20°C.

Preparation of Medium: Add components, ex

cept cefoperazone solution, amphotericin B solution,

and rifampicin solution to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 10.0mL of sterile cefoperazone solution, 10.0mL

of sterile amphotericin B solution, and 10.0mL of

sterile rifampicin solution. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Campylobacter species.

For the recovery of injured Campylobacter spp. from

foods.

<bdo id='7vfng'><sup id='7vfng'><div id='7vfng'><bdo id='7vfng'></bdo></div></sup></bdo>

在線客服

Modified Campylobacter Blood-Free Selective Agar Base (Modified Campylobacter Charcoal Differential Agar) (Modified CCDA) (BAM M30a)