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培養基配方

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MacConkey Agar, Fluorocult (Fluorocult MacConkey Agar)

[所屬分類:培養基配方] [發布時間:2021-11-30] [發布人:網站管理員2] [閱讀次數:] [返回]

MacConkey Agar, Fluorocult

(Fluorocult MacConkey Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Peptone from casein..........................................17.0g

Agar ..................................................................13.5g

Lactose..............................................................10.0g

NaCl....................................................................5.0g

Peptone from meat..............................................3.0g

Bile salt mixture..................................................1.5g

4-Methylumbelliferyl-β-D-glucuronide ..............0.1g

Neutral Red.......................................................0.03g

Crystal Violet ..................................................0.001g

pH 7.1 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Pour into sterile Petri

dishes. The plates are clear and red to red-brown.

Use: For the isolation of Salmonella, Shigella, and

coliform bacteria, in particular E. coli, from various

materials. The bile salts and crystal violet largely in

hibit the growth of Gram-positive microbial flora.

Lactose together with the pH indicator neutral red are

used to detect lactose-positive colonies and E. coli

can be seen among these because of fluorescence un

der UV light.