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MacConkey Agar, CS (BAM M91)

[所屬分類:培養基配方] [發布時間:2021-11-30] [發布人:網站管理員2] [閱讀次數:] [返回]
MacConkey Agar, CS
(BAM M91)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Peptone .............................................................17.0g
Agar ..................................................................13.5g
Lactose..............................................................10.0g
NaCl....................................................................5.0g
Proteose peptone.................................................3.0g
Bile salts..............................................................1.5g
Neutral Red.......................................................0.03g
Crystal Violet...................................................1.0mg
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a prepared me
dium from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring until boil
ing. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation and differentiation of lac
tose-fermenting and nonfermenting Gram-negative
bacteria while also controlling the swarming of Pro
teus species, if present. Lactose-fermenting organ
isms appear as red to pink colonies. Lactose-nonfer
menting organisms appear as colorless or transparent
colonies.
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