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培養基配方
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Lysine Medium
[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
Lysine Medium
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Glucose .............................................................44.5g
Agar ..................................................................17.8g
KH2PO4.............................................................1.78g
Lysine..................................................................1.0g
MgSO4·7H2O....................................................0.89g
CaCl2·2H2O ....................................................0.178g
NaCl................................................................0.089g
Inositol ..............................................................0.02g
Calcium pantothenate ......................................2.0mg
Adenine..........................................................1.78mg
DL-Methionine................................................0.89mg
L-Histidine......................................................0.89mg
DL-Tryptophan................................................0.89mg
Thiamine·HCl ..................................................0.4mg
Pyridoxine........................................................0.4mg
Nicotinic acid...................................................0.4mg
FeSO4·7H2O...................................................0.22mg
p-Aminobenzoic acid.......................................0.2mg
Riboflavin ........................................................0.2mg
MnSO4·H2O.................................................0.035mg
ZnSO4·7H2O ................................................0.035mg
(NH4)2MoO4·4H2O......................................0.018mg
H3BO3 ...............................................................8.9μg
Biotin ................................................................2.0μg
Folic acid ..........................................................1.0μg
Potassium lactate (50% solution).................. 10.0mL
Lactic acid....................................................... 1.0mL
pH 4.8 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Preparation of Medium: Add potassium lactate
to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Add remaining compo
nents, except lactic acid. Mix thoroughly. Gently heat
while stirring and bring to boiling. Do not autoclave.
Cool to 50°C. Adjust pH to 4.8 by adding 1.0mL of
lactic acid. Pour into sterile Petri dishes. Dry the sur
face of the plates by incubation at 37°C for 24 hr.
Use: For the isolation, cultivation, and enumeration
of wild yeasts encountered in brewing. For the isola
tion and enumeration of wild yeasts encountered in
brewing. On this medium, pitching yeasts are sup
pressed.



