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Lysine Iron Agar, Edward and Fife (BAM M89)

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]
Lysine Iron Agar, Edward and Fife
(BAM M89)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................13.5g
L-Lysine.............................................................10.0g
Pancreatic digest of gelatin.................................5.0g
Yeast extract........................................................3.0g
Glucose ...............................................................1.0g
Ferric ammonium citrate.....................................0.5g
Na2S2O3·5H2O ..................................................0.04g
Bromcresol Purple ............................................0.02g
pH 6.7 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems and Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Distribute into tubes in 10.0mL volumes.
Autoclave for 12 min at 15 psi pressure–121°C. Al
low tubes to cool in a slanted position.
Use: For the cultivation and differentiation of mem
bers of the Enterobacteriaceae based on their ability
to decarboxylate lysine and to form H2S. Bacteria
that decarboxylate lysine turn the medium purple.
Bacteria that produce H2S appear as black colonies.
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