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培養基配方

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Lowenstein-Jensen Medium with Streptomycin

[所屬分類:培養基配方] [發布時間:2021-11-29] [發布人:網站管理員2] [閱讀次數:] [返回]

Lowenstein-Jensen Medium

with Streptomycin

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 161.0mL:

Potato starch......................................................30.0g

Asparagine ..........................................................3.6g

KH2PO4...............................................................2.4g

Magnesium citrate ..............................................0.6g

Malachite Green..................................................0.4g

MgSO4·7H2O....................................................0.24g

Homogenized whole egg .................................. 1.0L

Glycerol ........................................................ 12.0mL

Streptomycin solution................................... 10.0mL

Homogenized Whole Egg:

Composition per liter:

Whole eggs ...................................................... 18-24

Preparation of Homogenized Whole Egg:

Use fresh eggs, less than 1 week old. Scrub the shells

with soap. Let stand in a soap solution for 30 min.

Rinse in running water. Soak eggs in 70% ethanol for

15 min. Break the eggs into a sterile container. Ho

mogenize by shaking. Filter through four layers of

sterile cheesecloth into a sterile graduated cylinder.

Measure out 1.0L.

Streptomycin Solution:

Composition per 10.0mL:

Streptomycin....................................................0.1mg

Preparation of Streptomycin Solution: Add

streptomycin to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add glycerol to

600.0mL of distilled/deionized water. Mix thorough

ly. Add remaining components, except fresh egg

mixture. Mix thoroughly. Gently heat while stirring

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 1.0L

of homogenized whole egg and 10.0mL of sterile

streptomycin solution. Mix thoroughly. Distribute

into sterile screw-capped tubes. Place tubes in a

slanted position. Inspissate at 85°C (moist heat) for

45 min.

Use: For the cultivation and differentiation of Myco

bacterium species. Mycobacterium tuberculosis ap

pears as granular, rough, dry colonies. Mycobacterium

kansasii appears as smooth to rough photochromoge

nic colonies. Mycobacterium gordonae appears as

smooth yellow-orange colonies. Mycobacterium avi

um appears as smooth, colorless colonies. Mycobacte

rium smegmatis appears as wrinkled, creamy white

colonies. Also used for the cultivation and mainte

nance of Gordona species, Nocardia species, Rhodo

coccus species, and Tsukamurella paurometabolum.

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Lowenstein-Jensen Medium with Streptomycin