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LICNR Broth (Lysine Iron Cystine Neutral Red Broth)

[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]

LICNR Broth

(Lysine Iron Cystine Neutral Red Broth)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 500.0mL:

L-Lysine·HCl .....................................................10.0g

Mannitol..............................................................5.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Salicin .................................................................1.0g

Ferric ammonium citrate.....................................0.5g

L-Cystine .............................................................0.1g

Na2S2O3 ..............................................................0.1g

Neutral Red.....................................................0.025g

Novobiocin solution...................................... 10.0mL

pH 6.2 ± 0.2 at 25°C

Novobiocin Solution:

Composition per 10.0mL:

Novobiocin......................................................0.015g

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Continue boiling for 2–

3 min. Distribute into tubes in 10.0mL volumes. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 0.1mL of sterile novobio

cin solution to each tube. Mix thoroughly.

Use: For the rapid, presumptive detection of Salmo

nella in foods, food ingredients, and feed materials.

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