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培養基配方
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Levine EMB Agar (Levine Eosin Methylene Blue Agar) (Eosin Methylene Blue Agar, Levine) (LEMB Agar) (BAM M80)
[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]
Levine EMB Agar
(Levine Eosin Methylene Blue Agar)
(Eosin Methylene Blue Agar, Levine)
(LEMB Agar)
(BAM M80)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................15.0g
Lactose..............................................................10.0g
Peptone..............................................................10.0g
K2HPO4...............................................................2.0g
Eosin Y ...............................................................0.4g
Methylene Blue............................................0.065mg
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the isolation, cultivation, and differentia
tion of Gram-negative enteric bacteria based on lac
tose fermentation. Bacteria that ferment lactose,
especially the coliform bacterium Escherichia coli,
appear as colonies with a green metallic sheen or
blue-black to brown color. Bacteria that do not fer
ment lactose appear as colorless or transparent light
purple colonies.



