培養基配方

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Lee’s Agar

[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]

Lee’s Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................18.0g

Pancreatic digest of casein................................10.0g

Yeast extract......................................................10.0g

Lactose................................................................5.0g

Sucrose................................................................5.0g

CaCO3 .................................................................3.0g

K2HPO4...............................................................0.5g

Bromcresol Purple (0.2% solution) .............. 10.0mL

pH 7.0 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 10.0mL:

Bromcresol Purple ............................................0.02g

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, ex

cept Bromcresol Purple solution, to distilled/deion

ized water and bring volume to 990.0mL. Mix

thoroughly. Adjust pH to 7.0. Gently heat and bring

to boiling. Autoclave for 20 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile

Bromcresol Purple solution. Mix thoroughly. Pour

into sterile, chilled Petri dishes in 20–25mL vol

umes. Swirl flask while dispensing to evenly sus

pend CaCO3. Dry plates at 30°C for 18–24 hr before

use.

Use: For the isolation, cultivation, and enumeration

of Lactobacillus bulgaricus from yogurt.

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