培養基配方

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Lecithin Lipase Anaerobic Agar

[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................40.0g

Agar ..................................................................25.0g

Yeast extract........................................................5.0g

Glucose ...............................................................2.0g

NaCl....................................................................2.0g

KH2PO4...............................................................1.0g

Na2HPO4·12H2O.................................................5.0g

MgSO4·7H2O......................................................0.1g

Egg yolk emulsion ...................................... 100.0mL

pH 7.6 ± 0.2 at 25°C

Egg Yolk Emulsion:

Composition:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

Preparation of Egg Yolk Emulsion: Soak eggs

with 1:100 dilution of saturated mercuric chloride so

lution for 1 min. Crack eggs and separate yolks from

whites. Mix egg yolks with 1 chicken egg. Filter ster

ilize.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile egg yolk emulsion. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation, cultivation, and differentia

tion of Clostridium species based on lecithinase pro

duction and lipase production. Bacteria that produce

lecithinase appear as colonies surrounded by a zone

of insoluble precipitate. Bacteria that produce lipase

appear as colonies with a pearly iridescent sheen.