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培養基配方

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LBS Agar (Lactobacillus Selection Agar)

[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]

LBS Agar

(Lactobacillus Selection Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Sodium acetate·3H2O........................................25.0g

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Pancreatic digest of casein................................10.0g

KH2PO4...............................................................6.0g

Yeast extract........................................................5.0g

Ammonium citrate ..............................................2.0g

Polysorbate 80.....................................................1.0g

MgSO4 ............................................................0.575g

FeSO4 ..............................................................0.034g

MnSO4 ..............................................................0.12g

Acetic acid, glacial........................................ 1.32mL

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept acetic acid, to distilled/deionized water and

bring volume to 998.7mL. Mix thoroughly. Gently

heat and bring to boiling. Add glacial acetic acid.

Mix thoroughly. Gently heat while stirring and bring

to 90°–100°C for 2–3 min. Do not autoclave. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation, cultivation, and enu

meration of lactobacilli.