培養基配方

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LBE Medium

[所屬分類:培養基配方] [發布時間:2021-11-26] [發布人:網站管理員2] [閱讀次數:] [返回]

LBE Medium

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................10.0g

Pancreatic digest of casein................................10.0g

Yeast extract........................................................5.0g

Glucose solution ........................................... 10.0mL

50X medium E................................................ 4.0mL

pH 7.0 ± 0.2 at 25°C

Glucose Solution:

Composition per 100.0mL:

D-Glucose..........................................................20.0g

Preparation of Glucose Solution: Add D-glu

cose to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

50X Medium E:

Composition per liter:

K2HPO4, anhydrous........................................500.0g

Na(NH4)HPO4·4H2O ......................................175.0g

Citric acid·H2O ...............................................100.0g

MgSO4·7H2O....................................................10.0g

Preparation of 50X Medium E: Add compo

nents to 670.0mL of distilled/deionized water in the

following order: MgSO4·7H2O, citric acid·H2O,

K2HPO4, and Na(NH4)HPO4·4H2O. Mix thoroughly.

Bring volume to 1.0L with distilled/deionized water.

Preparation of Medium: Add components—ex

cept glucose solution and 50X medium E—to dis

tilled/deionized water and bring volume to 986.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add

10.0mL of sterile glucose solution and 4.0mL of ster

ile 50X medium E. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Escher

ichia coli.