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Lactose Gelatin Medium (BAM M75)

[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]
Lactose Gelatin Medium
(BAM M75)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Gelatin.............................................................120.0g
Tryptose ............................................................15.0g
Lactose..............................................................10.0g
Yeast extract......................................................10.0g
Phenol Red (1.0% in ethanol solution) ........... 5.0mL
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add tryptose, yeast ex
tract, and lactose to distilled/deionized water and
bring volume to 400.0mL. Mix thoroughly. Add gel
atin to distilled/deionized water and bring volume to
590.0mL Gently heat gelatin solution while stirring
and bring to 50°–60°C. Add Phenol Red. Mix the two
solutions together. Distribute into tubes in 10.0mL
volumes. Autoclave for 10 min at 15 psi pressure–
121°C. If medium is not used in 8 hr, deoxygenate by
heating to 50°–70°C for 2–3 hr prior to inoculation.
Use: For the cultivation of Clostridium perfringens.
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