99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開
個人中心 我的訂單 購物車
編號：
批號：
培養基配方
您現在的位置： 網站首頁 >> 培養基配方

Lactose Egg Yolk Milk Agar

[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]
Lactose Egg Yolk Milk Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1206.0mL:

Lactose..............................................................12.0g
Agar ....................................................................1.0g
Columbia blood agar base .......................... 800.0mL
Skim milk.................................................... 150.0mL
Egg yolk emulsion, 50%............................... 36.0mL
Inhibitor solution .......................................... 20.0mL
Neutral Red (1% solution)............................ 3.25mL
pH 7.0 ± 0.2 at 25°C
Columbia Blood Agar Base:
Composition per 800.0mL:
Agar ..................................................................15.0g
Pantone .............................................................10.0g
Bitone................................................................10.0g
NaCl....................................................................5.0g
Tryptic digest of beef heart.................................3.0g
Cornstarch...........................................................1.0g
Preparation of Columbia Blood Agar Base:
Add components to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat
until boiling.
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks..................................................11
Whole chicken egg ..................................................1
NaCl (0.9% solution).................................... 50.0mL
Preparation of Egg Yolk Emulsion, 50%:
Soak eggs with 1:100 dilution of saturated mercuric
chloride solution for 1 min. Crack eggs and separate
yolks from whites. Mix egg yolks with 1 chicken egg.
Measure 50.0mL of egg yolk emulsion and add to
50.0mL of 0.9% NaCl solution. Mix thoroughly. Fil
ter sterilize. Warm to 45°–50°C.
Inhibitor Solution:
Composition per 20.0mL:
Neomycin sulfate..............................................0.18g
NaN3 .................................................................0.24g
Preparation of Inhibitor Solution: Add neo
mycin sulfate and NaN3 to distilled/deionized water
and bring volume to 20.0mL. Mix thoroughly. Filter
sterilize.
Caution: Sodium azide is toxic. Azides also react
with metals and disposal must be highly diluted.
Preparation of Medium: Combine Columbia blood
agar base, lactose, agar, and Neutral Red and bring vol
ume to 1.0L. Adjust pH to 7.0. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 55°C. Filter sterilize
skim milk. To 1.0L of cooled, sterile agar mixture, asep
tically add 150.0mL of sterile skim milk, 36.0mL of
sterile egg yolk emulsion, 50%, and 20.0mL of sterile
inhibitor solution. Mix thoroughly. Pour into sterile Pet
ri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Clostri
dium species.
  版權所有：山東拓普生物工程有限公司 營銷服務熱線：　郵箱：
© 2016-2020 ALL RIGHTS RESERVED.　　
購物車 去頂部