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Lactobacillus Medium III

[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]
Lactobacillus Medium III

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................10.0g
Fructose...............................................................7.0g
Glucose ...............................................................7.0g
Maltose................................................................7.0g
Meat extract ........................................................5.0g
Sodium acetate·3H2O..........................................5.0g
Yeast extract........................................................5.0g
K2HPO4·3H2O.....................................................2.6g
Diammonium citrate ...........................................2.0g
Sodium gluconate ...............................................2.0g
L-Cysteine·HCl ...................................................0.5g
MgSO4·7H2O ......................................................0.1g
MnSO4·4H2O ....................................................0.05g
Tween 80......................................................... 1.0mL
pH 6.3 ± 0.2 at 25°C
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Adjust pH to 6.3. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Lactobacillus species.
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