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Lactobacillus Medium II (DSMZ Medium 93)

[所屬分類:培養基配方] [發布時間:2021-11-25] [發布人:網站管理員2] [閱讀次數:] [返回]
Lactobacillus Medium II
(DSMZ Medium 93)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Glucose .............................................................20.0g
Na-acetate .........................................................20.0g
Agar ..................................................................15.0g
Pancreatic digest of casein................................10.0g
Yeast extract........................................................5.0g
Meat extract ........................................................2.0g
KH2PO4...............................................................0.5g
K2HPO4...............................................................0.5g
MgSO4·7H2O ......................................................0.2g
DL-mevalonic acid..........................................30.0mg
FeSO4·7H2O...................................................10.0mg
MnSO4·2H2O ...................................................7.5mg
Ethanol.......................................................... 40.0mL
Tween 80......................................................... 1.0mL
pH 5.2 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the cultivation and maintenance of Lactoba
cillus fructivorans and Lactobacillus homohiochii.
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