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KF Streptococcus Broth

[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]
KF Streptococcus Broth

山(shan)東拓普(pu)生物工程有(you)限公司 培(pei)養基配方 //ljxinglong.com

Composition per liter:

Maltose..............................................................20.0g
Sodium glycerophosphate.................................10.0g
Yeast extract......................................................10.0g
NaCl....................................................................5.0g
Pancreatic digest of casein..................................5.0g
Peptic digest of animal tissue..............................5.0g
Lactose................................................................1.0g
Na2CO3 ...........................................................0.636g
NaN3 ...................................................................0.4g
Phenol Red......................................................0.018g
2,3,5-Triphenyltetrazolium
chloride solution..................................... 10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Caution: Sodium azide is toxic. Azides also react
with metals and disposal must be highly diluted.
Preparation of Medium: Add components, ex
cept 2,3,5-triphenyltetrazolium chloride solution, to
distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 2,3,5-triph
enyltetrazolium chloride solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the selective cultivation of fecal streptococ
ci.
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