培養基配方

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KF Streptococcus Agar

[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]

山東(dong)拓普生物工程有限公司(si) 培(pei)養基(ji)配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g

Maltose..............................................................20.0g

Sodium glycerophosphate.................................10.0g

Yeast extract......................................................10.0g

NaCl....................................................................5.0g

Pancreatic digest of casein..................................5.0g

Peptic digest of animal tissue..............................5.0g

Lactose................................................................1.0g

NaN3 ...................................................................0.4g

2,3,5-Triphenyltetrazolium

chloride solution ..................................... 10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

2,3,5-Triphenyltetrazolium Chloride Solu

tion:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium chloride ...................0.1g

Preparation of 2,3,5-Triphenyltetrazolium

Chloride Solution: Add 2,3,5-triphenyltetrazolium

chloride to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Filter sterilize.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add components, ex

cept 2,3,5-triphenyltetrazolium chloride solution, to

distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 2,3,5-triph

enyltetrazolium chloride solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the selective cultivation and enumeration

of fecal streptococci.

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