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HPC Agar (Heterotrophic Plate Count Agar) (m-HPC Agar)

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]
HPC Agar
(Heterotrophic Plate Count Agar)
(m-HPC Agar)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Gelatin...............................................................25.0g
Pancreatic digest of gelatin...............................20.0g
Agar ..................................................................15.0g
Glycerol ........................................................ 10.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium is available from BD Diag
nostic Systems.
Preparation of Medium: Add components, ex
cept glycerol, to distilled/deionized water and bring
volume to 990.0mL. Mix thoroughly. Gently heat
and bring to boiling. Add glycerol. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Pour into sterile Petri dishes.
Use: For the the cultivation and enumeration of mi
croorganisms by the membrane filter method and het
erotrophic plate count technique.
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