培養基配方

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HP Medium

[所屬分類:培養基配方] [發布時間:2021-11-23] [發布人:網站管理員2] [閱讀次數:] [返回]

HP Medium

山東拓(tuo)普生物工程有(you)限(xian)公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of soybean meal....................20.0g

Beef extract.......................................................10.0g

Yeast extract........................................................6.0g

Ammonium citrate..............................................5.0g

Tween 80.............................................................0.5g

MgSO4·7H2O......................................................0.2g

MnSO4·4H2O....................................................0.05g

FeSO4·7H2O......................................................0.04g

Glucose solution ........................................... 10.0mL

Tetracycline solution..................................... 10.0mL

Glucose Solution:

Composition per 100.0mL:

Glucose .............................................................10.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Tetracycline Solution:

Composition per 100.0mL:

Tetracycline.......................................................10.0g

Preparation of Tetracycline Solution: Add tet

racycline to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept glucose solution and tetracycline solution, to

distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile

glucose solution and tetracycline solution. Mix thor

oughly. Aseptically distribute into sterile tubes or

flasks.

Use: For the cultivation and enumeration of Leu

conostoc species.

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