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培養基配方

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Glutarate Medium

[所屬分類:培養基配方] [發布時間:2021-11-22] [發布人:網站管理員2] [閱讀次數:] [返回]

Glutarate Medium

山(shan)東拓(tuo)普生物(wu)工程有限公司培養基(ji)配方 //ljxinglong.com

Composition per liter:

Sodium glutarate.................................................2.6g

NaCl....................................................................1.0g

KCl......................................................................0.5g

MgCl2·6H2O .......................................................0.4g

NH4Cl ...............................................................0.25g

KH2PO4...............................................................0.2g

CaCl2·2H2O ......................................................0.15g

Resazurin .........................................................1.0mg

Rumen fluid .................................................. 20.0mL

NaHCO3 solution.......................................... 20.0mL

Na2S·9H2O solution...................................... 10.0mL

Trace elements solution SL-10 ....................... 1.0mL

pH 7.2 ± 0.2 at 25°C

Trace Elements Solution SL-10:

Composition per liter:

FeCl2·4H2O .........................................................1.5g

CoCl2·6H2O .................................................190.0mg

MnCl2·4H2O ................................................100.0mg

ZnCl2..............................................................70.0mg

Na2MoO4·2H2O .............................................36.0mg

NiCl2·6H2O ....................................................24.0mg

H3BO3 ..............................................................6.0mg

CuCl2·2H2O .....................................................2.0mg

HCl (25% solution)....................................... 10.0mL

Preparation of Trace Elements Solution SL-10:

Add FeCl2·4H2O to 10.0mL of HCl solution. Mix

thoroughly. Add distilled/deionized water and bring

volume to 1.0L. Add remaining components. Mix

thoroughly. Gas under 80% N2 + 20% CO2. Auto

clave for 15 min at 15 psi pressure–121°C.

NaHCO3 Solution:

Composition per 20.0mL:

NaHCO3 ..............................................................2.5g

Preparation of NaHCO3 Solution: Add

NaHCO3 to distilled/deionized water and bring vol

ume to 20.0mL. Mix thoroughly. Filter sterilize. Gas

under 80% N2 + 20% CO2.

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O........................................................0.36g

Preparation of Na2S·9H2O Solution: Add

Na2S·9H2O to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Gas under 100%

N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense

medium under 80% N2 + 20% CO2. Add components,

except NaHCO3 solution and Na2S·9H2O solution, to

distilled/deionized water and bring volume to

970.0mL. Mix thoroughly. Sparge with 80% N2 + 20%

CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Aseptically and anaerobically add 20.0mL of sterile

NaHCO3 solution and 10.0mL of sterile Na2S·9H2O

solution. Mix thoroughly. Aseptically and anaerobi

cally distribute into sterile tubes or flasks.

Use: For the cultivation of a wide variety of bacteria.

that can utilize glutarate as a carbon source.

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Glutarate Medium