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Glutamine Medium

[所屬分類:培養基配方] [發布時間:2021-11-22] [發布人:網站管理員2] [閱讀次數:] [返回]
Glutamine Medium

山東(dong)拓普生物(wu)工程有限(xian)公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g
Pancreatic digest of gelatin.................................5.0g
Beef extract.........................................................3.0g
Glutamine/tryptophan solution ..................... 10.0mL
pH 6.8 ± 0.2 at 25°C
Glutamine/Tryptophan Solution:
Composition per liter:
L-Glutamine .................................................100.0mg
L-Tryptophan ...............................................100.0mg
Preparation of Medium: Add components, ex
cept glutamine/tryptophan solution, to distilled/
deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat while stirring and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 10.0mL of sterile glutamine/
tryptophan solution. Mix thoroughly. Pour into ster
ile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Escherichia coli.
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