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培養基配方

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Gluconate Peptone Broth

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]

Gluconate Peptone Broth

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Potassium gluconate .........................................40.0g

Casein peptone....................................................1.5g

K2HPO4...............................................................1.0g

Yeast extract........................................................1.0g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of Gram

negative bacteria based on their ability to oxidize

gluconate to 2-ketogluconate. For the differentiation

of fluorescent Pseudomonas species. After inocula

tion with bacteria and 48 hr of growth in this medi

um, Benedict’s reagent is added. Bacteria that

produce the reducing sugar 2-ketogluconate turn the

reagent yellow-orange to orange-red.