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培養基配方

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Giolitti-Cantoni Broth (Giolitti-Cantoni Broth Base with Tellurite)

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]

Giolitti-Cantoni Broth

(Giolitti-Cantoni Broth Base

with Tellurite)

山東(dong)拓普生(sheng)物工程有限公司培養基配方 //ljxinglong.com

Composition per 1030.0mL:

Mannitol............................................................20.0g

Pancreatic digest of casein................................10.0g

Beef extract.........................................................5.0g

LiCl .....................................................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Sodium pyruvate.................................................3.0g

Glycine................................................................1.2g

Chapman tellurite solution.............................. 0.3mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid and BD Diagnostic Systems.

Chapman Tellurite Solution:

Composition per 100.0mL:

K2TeO3 ................................................................3.5g

Preparation of Chapman Tellurite Solution:

Add K2TeO3 to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Caution: Potassium tellurite is toxic.

Preparation of Medium: Add components, ex

cept Chapman tellurite solution, to distilled/deion

ized water and bring volume to 1.0L. Gently heat and

bring to boiling. Mix thoroughly. Distribute into

tubes in 10.0mL volumes. Autoclave for 15 min at 15

psi pressure–121°C. Cool rapidly to 25°C. Immedi

ately prior to use, incubate tubes at 100°C to expel

oxygen. Aseptically add 0.3mL of sterile Chapman

tellurite solution to each tube. Mix thoroughly. Cool

to 25°C.

Use: For the cultivation and enrichment of Staphylo

coccus aureus from foods.

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Giolitti-Cantoni Broth (Giolitti-Cantoni Broth Base with Tellurite)