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培養基配方

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Fraser Broth

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]

Fraser Broth

山(shan)東(dong)拓普生物工程有(you)限公司培養基(ji)配方 //ljxinglong.com

Composition per liter:

NaCl................................................................. 20.0g

Na2HPO4.......................................................... 12.0g

Beef Extract ....................................................... 5.0g

Proteose peptone................................................ 5.0g

Pancreatic digest of casein................................. 5.0g

Yeast extract....................................................... 5.0g

LiCl.................................................................... 3.0g

KH2PO4............................................................ 1.35g

Esculin ...............................................................1.0g

Fraser supplement solution.......................... 10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Fraser Supplement Solution:

Composition per 10.0mL:

Ferric ammonium citrate.................................... 0.5g

Acriflavine·HCl ............................................... 0.25g

Nalidixic acid..................................................... 0.1g

Ethanol........................................................... 5.0mL

Preparation of Fraser Supplement Solution

Solution: Add components to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, ex

cept Fraser supplement solution, to distilled/deion

ized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile Fraser supplement

solution. Mix thoroughly. Aseptically distribute into

sterile tubes or flasks.

Use: For the isolation of Listeria species from foods.