培養基配方

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FPA Medium

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]

FPA Medium

山東拓(tuo)普生(sheng)物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Proteose peptone No. 3.................................... 20.0g

Agar ................................................................. 15.0g

Pectin, citrus ...................................................... 5.0g

K2HPO4.............................................................. 1.5g

MgSO4·7H2O..................................................... 1.5g

Antibiotic solution ....................................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide...............................................0.075g

Novobiocin ....................................................0.045g

Penicillin G ................................................. 75,000U

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except

agar and antibiotic solution, to distilled/deionized wa

ter and bring volume to 990.0mL. Mix thoroughly.

Adjust pH to 7.0. Add agar. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile antibiotic solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Pseudomo

nas species.