培養基配方

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Fish Peptone Agar

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

Fish Peptone Agar

山(shan)東拓普生物工(gong)程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ................................................................... 5.0g

Maltose .............................................................. 5.0g

NaCl................................................................... 5.0g

Peptone .............................................................. 5.0g

Pancreatic digest of casein................................. 5.0g

Yeast extract....................................................... 5.0g

Trout tissue extract solution......................... 50.0mL

pH 7.0 ± 0.2 at 25°C

Trout Tissue Extract Solution:

Composition per liter:

Fish (brook trout)...........................................500.0g

Pepsin................................................................. 1.0g

HCl, concentrated ........................................ 15.0mL

Preparation of Trout Tissue Extract: Add 1.0L

of distilled/deionized water to brook trout and blend

for 20–30 min. Add 1.0g of pepsin and 15.0mL of con

centrated HCl to digest the trout proteins. Incubate for

12 hr at 45°C. Adjust pH to 7.0. Allow solids to settle.

Filter sterilize. Do not autoclave. Store at 5°C.

Preparation of Medium: Add components, ex

cept trout tissue extract, to distilled/deionized water

and bring volume to 950.0L. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 13

psi pressure–118°C. Cool to 45°–50°C. Aseptically

add 50.0mL of sterile trout tissue extract. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation and maintenance of Aero

monas salmonicida.