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培養基配方

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FGTC Agar

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

FGTC Agar

山東拓普生物(wu)工(gong)程有限公(gong)司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein...............................15.0g

Agar .................................................................15.0g

Papaic digest of soybean meal...........................5.0g

NaCl...................................................................5.0g

KH2PO4..............................................................5.0g

Amylose Azure ..................................................3.0g

Galactose............................................................1.0g

Thallous acetate .................................................0.5g

MUG (4-Methylumbelliferyl-α-D-galactoside...0.1g

NaHCO3 solution ......................................... 20.0mL

Gentamicin solution....................................... 2.5mL

Tween 80...................................................... 0.75mL

pH 7.3 ± 0.2 at 25°C

Gentamicin Solution:

Composition per 10.0mL:

Gentamicin.......................................................0.01g

Preparation of Gentamicin Solution: Add

gentamicin to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly.

NaHCO3 Solution:

Composition per 20.0mL:

NaHCO3 .............................................................2.0g

Preparation of NaHCO3 Solution: Add the

NaHCO3 to distilled/deionized water and bring vol

ume to 20.0mL. Mix thoroughly. Filter sterilize. Use

freshly prepared solution.

Preparation of Medium: Add components, ex

cept NaHCO3 solution, to distilled/deionized water

and bring volume to 980.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add sterile NaHCO3 solution. Mix thoroughly.

Pour into sterile Petri dishes.

Use: For the cultivation, differentiation, and enu

meration of Enterococcus species based on starch hy

drolysis and production of fluorescence. Bacteria

that hydrolyze starch, such as Streptococcus bovis,

appear as colonies surrounded by a clear zone. Bac

teria that produce fluorescence, such as Streptococ

cus bovis and Enterococcus faecium, appear as

colonies surrounded by a zone of bright bluish fluo

rescence when viewed under a long-wave UV lamp.

Other bacteria, such as Enterococcus faecalis, En

terococcus avium, or Streptococcus equinus, do not

hydrolyze starch or produce fluorescence.

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FGTC Agar