99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開

編號：
批號：

培養基配方

您現在的位置：網站首頁 >> 培養基配方

Fermentation Broth (CHO Medium)

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

Fermentation Broth

(CHO Medium)

山東(dong)拓普生物工程(cheng)有限公司培養基(ji)配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein............................... 15.0g

Yeast extract....................................................... 7.0g

NaCl................................................................... 2.5g

Agar ................................................................. 0.75g

Sodium thioglycolate......................................... 0.5g

L-Cystine .......................................................... 0.25g

Ascorbic acid ..................................................... 0.1g

Bromthymol Blue ............................................ 0.01g

Carbohydrate or starch solution................. 100.0mL

pH 7.0 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate...................................................... 6.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Starch Solution:

Composition per 100.0mL:

Starch ................................................................. 2.5g

Preparation of Starch Solution: Add starch to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add 100.0mL of sterile carbohydrate solution.

Mix thoroughly. Aseptically distribute into sterile

tubes or flasks. Loosen caps on tubes. Place in an

anaerobic chamber under an atmosphere of 85% N2,

10% H2, and 5% CO2. Fasten the caps securely or

maintain in an anaerobic chamber.

Use: For the differentiation of anaerobic bacteria

based upon carbohydrate fermentation. Bacteria that

ferment carbohydrates turn the medium yellow.

在線客服

Fermentation Broth (CHO Medium)