培養基配方

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Fermentation Basal Medium

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

山(shan)東拓普生物工程(cheng)有限(xian)公司培(pei)養基配(pei)方 //ljxinglong.com

Composition per liter:

Agar .................................................................15.0g

(NH4)2HPO4.......................................................1.0g

MgSO4·7H2O .....................................................0.2g

KCl...................................................................0.02g

Carbohydrate solution................................ 100.0mL

Bromcresol Purple solution ......................... 20.0mL

pH 7.0 ± 0.2 at 25°C

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Bromcresol Purple Solution:

Composition per 100.0mL:

Bromcresol Purple ...........................................0.04g

Ethanol......................................................... 50.0mL

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to 50.0mL of absolute etha

nol. Add distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 100.0mL of sterile carbohydrate so

lution. Various carbohydrates are used for different

fermentation tests. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the differentiation of aerobic actinomycetes

based upon carbohydrate fermentation. Actino

mycetes that produce acid from carbohydrates turn

the medium yellow.