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FC Agar (Fecal Coliform Agar) (m-FC Agar) (m-Fecal Coliform Agar)

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

FC Agar

(Fecal Coliform Agar)

(m-FC Agar)

(m-Fecal Coliform Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar .................................................................15.0g

Lactose.............................................................12.5g

Tryptose ...........................................................10.0g

NaCl...................................................................5.0g

Proteose peptone No. 3 ......................................5.0g

Yeast extract.......................................................3.0g

Bile salts.............................................................1.5g

Aniline Blue.......................................................0.1g

Rosolic acid solution.................................... 10.0mL

pH 7.4 ± 0.2 at 25°C

Rosolic Acid Solution:

Composition per 100.0mL:

Rosolic acid........................................................1.0g

Preparation of Rosolic Acid Solution: Add ro

solic acid to 0.2N NaOH and bring volume to 100.0L.

Mix thoroughly.

Preparation of Medium: Add 10.0mL rosolic

acid solution to 950.0mL of distilled/deionized wa

ter. Mix thoroughly. Add other components and bring

volume to 1.0L with distilled/deionized water. Mix

thoroughly. Gently heat and bring to boiling with fre

quent mixing. Do not autoclave. Pour into sterile Pet

ri dishes or leave in tubes.

Use: For the enumeration of coliform bacteria using

the membrane filtration method.