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FC Agar (Fecal Coliform Agar) (m-FC Agar) (m-Fecal Coliform Agar)

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
FC Agar
(Fecal Coliform Agar)
(m-FC Agar)
(m-Fecal Coliform Agar)

山東拓普生物工程有限(xian)公司(si) 培養基配方 //ljxinglong.com

Composition per liter:

Agar ................................................................. 15.0g
Lactose.............................................................12.5g
NaCl................................................................... 5.0g
Proteose peptone No. 3 ......................................5.0g
Yeast extract.......................................................3.0g
Bile salts.............................................................1.5g
Aniline Blue.......................................................0.1g
Rosolic acid solution.................................... 10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Rosolic Acid Solution:
Composition per 100.0mL:
Rosolic acid........................................................1.0g
Preparation of Rosolic Acid Solution: Add ro
solic acid to 0.2N NaOH and bring volume to 100.0L.
Mix thoroughly.
Preparation of Medium: Add 10.0mL rosolic
acid solution to 950.0mL distilled/deionized water.
Mix thoroughly. Add other components and bring
volume to 1.0L with distilled/deionized water. Mix
thoroughly. Gently heat and bring to boiling with fre
quent mixing. Do not autoclave. Pour into sterile Pet
ri dishes or leave in tubes.
Use: For the enumeration of coliform bacteria using
the membrane filtration method.
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