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Entamoeba Medium (Endamoeba Medium)

[所屬分類:培養基配方] [發布時間:2021-11-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Entamoeba Medium
(Endamoeba Medium)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Liver infusion..................................................272.0g
Rice powder ......................................................14.2g
Agar ..................................................................11.0g
Proteose peptone.................................................5.5g
Sodium glycerophosphate...................................3.0g
NaCl....................................................................2.7g
Horse serum .................................................. 50.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systemss.
Rice Powder:
Composition per 15.0g:
Rice powder ......................................................15.0g
Preparation of Rice Powder: Sterilize rice pow
der at 160°C for 60 min. Do not overheat or rice pow
der will scorch.
Preparation of Medium: Add components, ex
cept horse serum and rice powder, to distilled/deion
ized water and bring volume to 994.0mL. Mix
thoroughly. Gently heat and bring to boiling. Distrib
ute into tubes in 7.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C. Allow tubes to cool in
a slanted position. Aseptically add enough sterile
horse serum to each tube to cover about half the slant.
Aseptically add 0.1g of sterile rice powder to each
tube.
Use: For the cultivation of Entamoeba histolytica.
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