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Eagle’s Minimal Essential Medium with Earle’s Salts and Nonessential Amino Acids (MEM with Earle’s Salts and Nonessential Amino Acids) (BAM M46)

[所屬分類:培養基配方] [發布時間:2021-11-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Eagle’s Minimal Essential Medium
with Earle’s Salts
and Nonessential Amino Acids
(MEM with Earle’s Salts
and Nonessential Amino Acids)
(BAM M46)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaCl....................................................................6.8g
NaHCO3..............................................................2.2g
Glucose ...............................................................1.0g
KCl......................................................................0.4g
CaCl2·2H2O ....................................................0.265g
MgSO4·7H2O......................................................0.2g
L-Arginine·H2O.................................................0.15g
NaH2PO4·H2O...................................................0.14g
L-Arginine·HCl................................................0.126g
L-Lysine·HCl ..................................................72.5mg
L-Tyrosine, disodium salt...............................52.1mg
L-Leucine .......................................................52.0mg
L-Threonine....................................................48.0mg
L-Valine ..........................................................46.0mg
L-Histidine·HCl·H2O......................................42.0mg
D-Phenylalanine .............................................32.0mg
L-Cysteine·2HCl...........................................31.29mg
L-Methionine..................................................15.0mg
L-Glutamic acid..............................................14.7mg
L-Aspartic acid ...............................................13.3mg
L-Proline.........................................................11.5mg
L-Serine ..........................................................10.5mg
L-Tryptophan..................................................10.0mg
L-Alanine..........................................................8.9mg
Phenol Red.....................................................10.0mg
L-Glycine..........................................................7.5mg
i-Inositol...........................................................2.0mg
D-Calcium pantothenate ...................................1.0mg
Choline chloride...............................................1.0mg
Folic acid .........................................................1.0mg
Nicotinamide....................................................1.0mg
Pyridoxal·HCl ..................................................1.0mg
Thiamine·HCl ..................................................1.0mg
Riboflavin ........................................................0.1mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to
1.0L of distilled/deionized water. Mix thoroughly.
Filter sterilize.
Use: For the cultivation of animal cells in tissue cul
ture, for example cells for viral detection and identi
fication by characteristic cytopathic effects.
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