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培養基配方

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E Agar (m-E Agar)

[所屬分類:培養基配方] [發布時間:2021-11-16] [發布人:網站管理員2] [閱讀次數:] [返回]

E Agar

(m-E Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Yeast extract......................................................30.0g

Agar ..................................................................15.0g

NaCl..................................................................15.0g

Pancreatic digest of gelatin...............................10.0g

Esculin ................................................................1.0g

Nalidixic acid....................................................0.25g

NaN3 .................................................................0.15g

Cycloheximide..................................................0.05g

TTC solution................................................. 15.0mL

pH 7.1 ± 0.2 at 25°C

TTC Solution:

Composition per 15.0mL:

2,3,5-Triphenyltetrazolium chloride .................0.15g

Preparation of TTC Solution: Add triphenyltet

razolium chloride to distilled/deionized water and

bring volume to 15.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, ex

cept TTC solution, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

sterile TTC solution. Mix thoroughly. Pour into ster

ile Petri dishes or distribute into sterile tubes.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Use: For the isolation, cultivation, and enumeration

of enterococci in water by the membrane filter meth

od. It is used in conjunction with esculin iron agar.