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Decarboxylase Basal Medium (BAM M44)

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]

Decarboxylase Basal Medium

(BAM M44)

山(shan)東拓(tuo)普生物工程有(you)限公(gong)司(si) 培(pei)養(yang)基(ji)配(pei)方 //ljxinglong.com

Composition per liter:

Peptone or gelysate.............................................5.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Bromcresol Purple ............................................0.02g

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Adjust pH so that it will be 6.5 ± 0.2 after

sterilization. Distribute into 16 × 150mm screw

capped tubes in 5.0mL volumes. Autoclave medium

with loosely capped tubes for 10 min at 15 psi pressure–

121°C. Screw the caps on tightly for storage and after

inoculation.

Use: For the cultivation and differentiation of bacte

ria based on their ability to decarboxylate the amino

acid. As the basal medium for arginine broth, lysine

broth, and ornithine broth. Bacteria that decarboxy

late arginine, lysine, or ornithine turn the medium

turbid purple. The unsupplementd decarboylase bas

al medium is used as a control.

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