培養基配方

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Czapek Yeast Extract Agar

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物(wu)工程有限(xian)公司培養基配(pei)方(fang) //ljxinglong.com

Composition per liter:

Sucrose..............................................................30.0g

Agar ................................................................. 15.0g

Yeast extract........................................................5.0g

K2HPO4...............................................................1.0g

Czapek concentrate ....................................... 10.0mL

Czapek Concentrate:

Composition per liter:

NaNO3.............................................................. 30.0g

KCl..................................................................... 5.0g

MgSO4·7H2O ..................................................... 5.0g

FeSO4·7H2O ...................................................... 0.1g

ZnSO4·7H2O ...................................................... 0.1g

CuSO4·5H2O .................................................... 0.05g

Preparation of Czapek Concentrate: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation and maintenance of As

pergillus niger, which is used for the production of

food enzymes and citric acid and a common food

contaminant.