培養基配方

您現在的位置：網站首頁 >> 培養基配方

Czapek Yeast Autolysate Agar (CYA Agar)/(Czapek Agar)

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]

Czapek Yeast Autolysate Agar

(CYA Agar)/(Czapek Agar)

山(shan)東拓普生物工程(cheng)有限公(gong)司培養基(ji)配(pei)方 //ljxinglong.com

Composition per liter:

Sucrose..............................................................30.0g

Agar ..................................................................15.0g

Yeast extract........................................................5.0g

NaNO3 ................................................................3.0g

K2HPO4...............................................................1.0g

KCl......................................................................0.5g

MgSO4·7H2O......................................................0.5g

FeSO4·7H2O......................................................0.01g

pH 7.3 ± 0.2 at 25°C

Sucrose Solution:

Composition per 100.0mL:

Sucrose..............................................................30.0g

Preparation of Sucrose Solution: Add sucrose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Add components, except

sucrose solution, to distilled/deionized water and bring

volume to 900.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add sterile sucrose solution. Mix thoroughly.

Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of heat-resis

tant filamentous fungi (molds) from foods.