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培養基配方
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Cooked Meat Medium, Modified (BAM M43)
[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Cooked Meat Medium, Modified
(BAM M43)
山東拓(tuo)普生物工程有限公司 培養(yang)基(ji)配(pei)方 //ljxinglong.com
Composition per tube:
Cooked meat medium .........................................1.0g
Diluent ............................................................... 1.0L
pH 6.8 ± 0.2 at 25°C
Cooked Meat Medium:
Composition per 481g:
Beef heart........................................................454.0g
Proteose peptone...............................................20.0g
NaCl....................................................................5.0g
Glucose ...............................................................2.0g
Source: Cooked meat medium is available in dehy
drated form from BD Diagnostic Systems.
Diluent:
Composition per liter:
Pancreatic digest of casein................................10.0g
Glucose ...............................................................2.0g
Soluble starch......................................................1.0g
Sodium thioglycolate..........................................1.0g
Neutral Red (1% aqueous).............................. 5.0mL
Preparation of Diluent: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat until dissolved.
Preparation of Medium: Add 1.0g of dehydrated
cooked meat medium and 15.0mL diluent to 20 ×
150mm test tubes. Let meat particles rehydrate. Gen
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C.
