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Cooked Meat Medium, Modified

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Cooked Meat Medium, Modified

山東拓普生(sheng)物工程有限(xian)公司 培(pei)養基(ji)配方 //ljxinglong.com

Composition per liter:

Cooked meat medium .......................................66.0g
Solution A.......................................................... 1.0L
pH 6.8 ± 0.2 at 25°C
Solution A:
Composition per liter:
Pancreatic digest of casein................................10.0g
Glucose ...............................................................2.0g
Soluble starch......................................................1.0g
Sodium thioglycolate ..........................................1.0g
Neutral Red (1% aqueous).............................. 5.0mL
Preparation of Solution A: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat until dissolved.
Preparation of Medium: Add 1.0g of cooked meat
medium to each of 66 test tubes. Add 15.0mL of solu
tion A to each test tube. Allow meat particles to rehy
drate. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of a variety of anaerobic mi
croorganisms.
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