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Cooked Meat Medium (BAM M42)

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Cooked Meat Medium
(BAM M42)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Beef heart........................................................454.0g
Proteose peptone...............................................20.0g
NaCl....................................................................5.0g
Glucose ...............................................................2.0g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Finely chop beef heart.
Add approximately 1.5g of heart particles to test tubes.
Add remaining components to distilled/deionized wa
ter and bring volume to 1.0L. Mix thoroughly. Distrib
ute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C. Slowly cool tubes to
prevent expulsion of meat particles. Steam the steril
ized medium and cool, without agitation, just before
use.
Use: For the cultivation and maintenance of anaero
bic microorganisms.
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