培養基配方

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Clostridium aminobutyricum Medium

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普(pu)生物工程有限公司培(pei)養基配方 //ljxinglong.com

Composition per liter:

K2HPO4...............................................................7.0g

γ-Aminobutyric acid ...........................................5.0g

Yeast extract........................................................3.0g

Agar ....................................................................1.5g

KH2PO4...............................................................1.3g

MgCl2·6H2O .......................................................0.2g

CaCl2·2H2O.......................................................0.01g

FeCl3·6H2O .......................................................0.01g

Na2MoO4·2H2O ...............................................1.0mg

Resazurin .........................................................1.0mg

NaHCO3 solution .......................................... 20.0mL

Na2S·9H2O solution ...................................... 20.0mL

pH 7.2 ± 0.2 at 25°C

NaHCO3 Solution:

Composition per 20.0mL:

NaHCO3 ..............................................................1.0g

Preparation of NaHCO3 Solution: Add

NaHCO3 to distilled/deionized water and bring vol

ume to 20.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Na2S·9H2O Solution:

Composition per 20.0mL:

Na2S·9H2O..........................................................0.3g

Preparation of Na2S·9H2O Solution: Add

Na2S·9H2O to distilled/deionized water and bring

volume to 20.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Preparation of Medium: Add components, ex

cept NaHCO3 solution and Na2S·9H2O solution, to

distilled/deionized water and bring volume to

960.0mL. Mix thoroughly. Sparge under 80% N2 +

20% CO2. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically and anaerobically add 20.0mL of

sterile NaHCO3 solution and 20.0mL of sterile

Na2S·9H2O solution. Mix thoroughly.

Use: For the growth and maintenance of Clostridium

aminobutyricum and other Clostridium species